Histology Photo Gallery

Masson's Trichrome  -  Demonstration of collagenous connective tissue, cytoplasm and nuclei

 Periodic Acid Schiff Stain for fungus (PAS-F)

• 1% Periodic Acid 
• Schiff reagent 
• Hematoxylin
• Bluing reagent

Gram B & B - Demonstration of Gram positive and Gram negative bacteria, for example Staphylococcus aureus (+) and E. coli (-)

• Acetone
• Crystal Violet solution
• Gram's Iodine solution
• Safranin O solution
• Tartrazine solution

In the photo, clusters of Gram positive can be seen in violet and Gram negative can be seen in scattered red dots throughout the specimen.

Acid Fast Bacilli - Zeihl Nielsen 

Demonstration of mycobacteria, most notably M. tuberculosis

• Carbon Fuchsin solution
• 1% Acid Alcohol
• Methylene Blue solution

Verhoeff-Van Gieson - Demonstration of elastic fibers

• Weigert's iodine solution
• 10% aqueous ferric chloride
• 5% alcholic hematoxylin
• 5% aqueous sodium thiosulfate
• Van Gieson's solution